Characterization of Ca2+ Transport in Purified Endoplasmic Reticulum Membrane Vesicles from Lepidium sativum L. Roots
نویسندگان
چکیده
منابع مشابه
Enzymic Properties of Purified Myrosinase from Lepidium sativum Seedlings
To establish the substrate specificity o f the thioglucoside glucohydrolase myrosinase (EC 3.2.3.1), this enzyme was purified to homogeneity from light-grown cress (Lepidium sativum L.) seedlings by Sephadex gel filtration. Red Dye and anion exchange (FPLC M ono Q) chro matography, and preparative isoelectric focusing. Hydrolytic activity was shown toward only 4 o f the 29 synthetic and natura...
متن کاملCharacteristics of endoplasmic reticulum-derived transport vesicles
We have isolated vesicles that mediate protein transport from the ER to Golgi membranes in perforated yeast. These vesicles, which form de novo during in vitro incubations, carry lumenal and membrane proteins that include core-glycosylated pro-alpha-factor, Bet1, Sec22, and Bos1, but not ER-resident Kar2 or Sec61 proteins. Thus, lumenal and membrane proteins in the ER are sorted prior to transp...
متن کاملCalcium and proton transport in membrane vesicles from barley roots.
Ca(2+) uptake by membrane fractions from barley (Hordeum vulgare L. cv CM72) roots was characterized. Uptake of (45)Ca(2+) was measured in membrane vesicles obtained from continuous and discontinuous sucrose gradients. A single, large peak of Ca(2+) uptake coincided with the peak of proton transport by the tonoplast H(+)-ATPase. Depending on the concentration of Ca(2+) in the assay, Ca(2+) upta...
متن کاملCoated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.
The G protein of vesicular stomatitis virus is a transmembrane glycoprotein that is transported from its site of synthesis in the rough endoplasmic reticulum to the plasma membrane via the Golgi apparatus. Clathrin-coated vesicles have been purified from CHO cells infected with vesicular stomatitis virus and shown to contain G protein in amounts nearly stoichiometric with clathrin. Pulse-chase ...
متن کاملProtein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane.
We have reproduced the process of protein transport across and of protein integration into the mammalian endoplasmic reticulum membrane by the use of proteoliposomes reconstituted from pure phospholipids and purified membrane proteins. The transport of some proteins requires only two membrane protein complexes: the signal recognition particle receptor, needed for targeting of a nascent chain to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1984
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.76.4.962